Wednesday, February 5, 2014

Enzymes

ENZYMES INTRODUCTION: Enzymes are proteins produced by living cells. They act as biochemical catalysts meaning they lower the activation energy required for a biochemical reception to occur. A substratum, is the substance to be acted upon, it binds to the active put of an enzyme to induce the biochemical reaction and deposit the desired product. Each active site of the enzyme is unique to the substratum it pass on bind with. However, the activeness of an enzyme can be raise by many factors such as pH and temperature and social movement line of inhibitors be wee-wee these factors can alter the structure of the enzyme and denature it. When the temperature and pH are higher or lower than what is optimal for the enzyme to work, this when the enzymes performance will be affected; it can each be disabled or slowed down. However the inhibitor binds to the active site of an enzyme and tug it, which will prevent the substrate to bind to it, and thence the reaction w ill slow down. In this lab we will running the effects of enzymatic activity by conducing 3 look intos, to con how changes in the temperature, pH, and presence of inhibitors will cause this effect. EXPERIMENT 1 Enzyme activity: the effect of temperature. Materials: Potato educe Catechol 5 analyse vacuum tobacco pipes Test resistance pallbearer Ice can Warm bath Boiling bath Pen Ruler Procedure: 1.With pen and formula recognize 1cm and 2cm from bottom on each 5 test tubes. 2. come across tube 1 till 1cm mark with distilled water. 3.Fill test tubes 2, 3, 4, 5 with potato extract till 1 cm mark. 4.Place tube 2 in stewing water bath. 5.Place tube 3 in speedy water bath. 6.Place tube 4 in sparkler bath. 7.Place tube 5 in test tube rack, on room temperature. 8.Leave the tubes for 5 minutes. 9.Add catechol in all 5 tubes up till the 2cm mark. 10.Observe the simulation change for the next 20 minutes. Observation and results: In this experiment w e can identify: The substrate: catechol T! he enzyme: catechol oxidase The...If you want to get a full essay, order it on our website: OrderCustomPaper.com

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